Figure 5 | Oncogene

Figure 5

From: The Snail repressor recruits EZH2 to specific genomic sites through the enrollment of the lncRNA HOTAIR in epithelial-to-mesenchymal transition

Figure 5

Snail binding sites chromatin trimethylation requires HOTAIR. (a) qPCR analysis of ChIP assays with anti-Snail antibody (IP Snail) and, as control, normal rabbit IgG (IgG) on chromatin from murine hepatocyte cells silenced for HOTAIR (siHotair) or GFP (siCtr), as control, and treated (+TGFβ) or not (−TGFβ) with TGFβ for 24 h. Data show the direct recruitment of endogenous Snail on the correspondent consensus binding sites on the murine promoters of HNF4α, E-cadherin and HNF1α. Timm promoter was used as a negative control. Values derived from five independent experiments are reported as means ±s.e.m. and expressed as percentage of the Input chromatin (% Input). Statistically significant differences are reported (*P<0.05, **P<0.01, ***P<0.001). (b) qPCR analysis of ChIP assays with anti-H3K27me3 antibody (IPH3K27me3) and, as controls, normal rabbit IgG (IgG) on chromatin from murine hepatocyte cells silenced for HOTAIR (siHotair) or GFP (siCtr), as control, and treated (+TGFβ) or not (−TGFβ) with TGFβ for 24 h. Data show the enrichment of H3K27 trimethylation on the Snail consensus binding sites on the murine promoters of HNF4α, E-cadherin and HNF1α, as above. Timm promoter was used as a negative control. Values derived from five independent experiments are reported as means ± s.e.m. and expressed as the percentage of the Input chromatin (% Input). Statistically significant differences are reported (*P<0.05, **P<0.01).

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