Figure 6 | Oncogene

Figure 6

From: The Snail repressor recruits EZH2 to specific genomic sites through the enrollment of the lncRNA HOTAIR in epithelial-to-mesenchymal transition

Figure 6

EZH2 is recruited by Snail to its target genes by means of HOTAIR. (a) qPCR analysis of ChIP assays with an anti-EZH2 antibody (IP EZH2) and, as controls, normal rabbit IgG (IgG) on chromatin from murine hepatocyte cells silenced for HOTAIR (siHotair) or GFP (siCtr), as control, and treated (+TGFβ) or not (−TGFβ) with TGFβ for 24 h. Data show the recruitment of EZH2 on the Snail consensus binding sites on the murine promoters of HNF4α, E-cadherin and HNF1α and, as control, its displacement from the HNF4α binding site on Snail promoter.29 Timm promoter was used as unresponsive control sequence. Values derived from five independent experiments are reported as means ± s.e.m. and expressed as the percentage of the Input chromatin (% Input). Statistically significant differences are reported (*P<0.05; **P<0.01; ***P<0.001). (b) qPCR analysis of ReChIP assays of samples TGFβ treated for 24 h and silenced for HOTAIR (siHotair) or GFP (siCtr), as control. Values, derived from three independent experiments, are reported as means ±s.e.m. and expressed as the percentage of the Input chromatin (% Input).

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