Figure 4
The distributions of NCL and MMP7 in lung cancer cells in vitro and in vivo. (a) The 5-μm slices of paraffin-embedded specimens were prepared for IHC and immunoflurescent staining by using anti-c23 and anti-MMP7 antibodies. (b) NCL and MMP7 expression levels in lung tumor tissues of K-rasG12D lung cancer mice. Two consecutive sections were stained at different times to confirm indicated protein expression in the lung. (c) Fixed H1299 and A549 cells were immunofluorescently co-stained by antibodies targeting NCL and MMP7. (d) The whole cell (W), cytosolic (Cy), nuclear (Nu), nucleoplasmic and nucleolar fractions were prepared for western blotting.
