Figure 3 | Oncogene

Figure 3

From: Erratum: Aberrant Mer receptor tyrosine kinase expression contributes to leukemogenesis in acute myeloid leukemia

Figure 3

Merstimulation in AML cells activates oncogenic signaling pathways. Kasumi-1 and NOMO-1 cells were incubated in serum-free RPMI medium for two to three hours and then treated with 200nM rhGas6 or buffer. (c) Diluted lysates were incubated with human phospho-kinase array membranes and bound phospho-proteins were detected according to kit instructions. Each membrane contains positive control (P) antibodies spotted in duplicate. Proteins that demonstrated a 1.5-fold or greater increase in phosphorylation after stimulation with Gas6 (Gas6 treated) relative to buffer treated samples (Untreated) are marked by numbers between duplicate spots, which correlate with the identification numbers shown in (d). (e) Cell lysates were prepared from cultures treated with rhGas6 (+) or buffer (−) and subjected to immunoblot analysis with antibodies specific for phosphorylated and total MSK1, p38, ERK1/2, STAT6, AKT, ATF1, and CREB proteins. Representative Kasumi-1 immunoblots are shown.

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