Figure 4
UBR5 knockdown enhances apoptosis dependent on MOAP-1. (a) A2780 cells were transfected with siCtrl, siUBR5, siMOAP-1 or siUBR5 and siMOAP-1. Cell lysates were prepared 48 h posttransfection and immunoblotted as indicated. n=3 independent experiments. (b) A2780 cells were prepared as described in panel (a) and treated with or without 10 μM of cisplatin for 48 h and stained with Alexa Fluor 488-conjugated Annexin V for flow cytometric analysis. Percentage of apoptotic cells is indicated as Annexin V-positive cells. n=4 independent experiments (means+s.e.m.). *P<0.05 by unpaired two-tailed t-test. (c) A2780 cells were transfected as in panel (a) and treated with or without 10 μM of cisplatin for 12 h. Cell lysates were used for IP with active Bax-specific antibody (6A7). IP samples were immunoblotted with total Bax antibody. n=3 independent experiments.
