Figure 2 | Oncogene

Figure 2

From: Pathobiological implications of the d16HER2 splice variant for stemness and aggressiveness of HER2-positive breast cancer

Figure 2

Molecular analyses of d16HER2- and WTHER2-positive cell lines. (a) Dendrogram obtained from hierarchical clustering of MI6, MI7, WTHER2_1 and WTHER2_2 tumor cell lines based on the expression of the top 5000 most variable genes. (b) Volcano plot of log2 fold changes vs –log10 false discovery rate (FDR) showing transcriptional differences between d16HER2 and WTHER2 cells. Vertical dashed lines represent the 1.5-fold change cut-off and the horizontal dashed line denotes the 0.05 FDR cut-off. Up- and downregulated genes are highlighted in red and green, respectively. (c) Bar plot showing significantly enriched KEGG and REACTOME pathways in genes upregulated in MI6 and MI7 (orange bars) or WTHER2_1 and WTHER2_2 cells (green bars). (d) GSEA enrichment plots of Wnt and Notch gene sets in d16HER2 cells compared with WTHER2. NES, normalized enrichment score. (e) Heatmap showing the expression in d16HER2 and WTHER2 cells of differentially expressed genes included in the Wnt and Notch gene sets.

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