Figure 6

Differential expression of CD44 in engineered human BC cell lines. (a, b) Representative FACS analysis of CD44 expression levels in HER2-positive cell subsets of MCF7_d16 and MCF7_WT and in bulk MCF-Mock (a) and in T47D_d16, and T47D_WT and in bulk T47D-Mock-infected cells (b). (c, d) Representative immunofluorescence of expression and co-expression (merged) of HER2 (red) and CD44 (blue) markers in the HER2-positive cell subsets of MCF_d16 and MCF_WT cells (c) and of T47D_d16 and T47D_WT-infected cells (d) were evaluated by confocal microscopy. The nuclei were counterstained with DAPI-Prolong (cyan). (e, f) Box plot showing CD44 fluorescence intensity in HER2-positive cell subsets of MCF7_d16, MCF7_WT and in bulk MCF7-Mock (e) and in T47D_d16, T47D_WT and in bulk T47D-Mock-infected cells (f). Significance was calculated by a two-tailed unpaired t-test.