Figure 5

The DDR prevents Aurora A recruitment to the Plk1/Bora complex. Just before mitotic entry in G2 Aurora A is recruited to the Plk1/Bora complex and phosphorylates Plk1 at T210 (panel 1). During DNA damage, recruitment of Aurora A to the Plk1/Bora complex is prevented (panel 2). Expression of a constitutively active Aurora A-T288E mutant is unable to phosphorylate Plk1 at T210 (panel 3). Forced association of Aurora A to Bora by fusing both proteins together during the DDR results in phophorylation of Plk1 at T210 and subsequent activation (panel 4).