Figure 4

A1 knockdown leads to the loss of leukemic B cells. (a) Quantification of B cells in the spleen, bone marrow, blood and axillary lymph nodes of diseased mice. Single cell suspensions were stained with anti-B220 antibodies. Individual bars depict the percentage of B220⁺ B cells from the total cell fraction of MYC or DT-FF mice (>90% Venus⁺ cells) as well as of Venus⁻ (DT-A1⁻) and Venus⁺ (DT-A1⁺) cells from DT-A1 mice. Bars represent means±s.e.m. (MYC n=4, DT-FF n=5, DT-A1 n=6, biological replicates). ANOVA followed by Bonferroni post hoc test. *P⩽0.05, **P⩽0.01, ***P⩽0.001. (b) Analysis of leucocyte subset distribution in Venus⁺ (DT-A1⁺) and Venus⁻ (DT-A1⁻) cell fractions of premalignant 4–6-week-old DT-A1 mice in comparison to Eμ-MYC control mice. LN=lymph nodes, BM=bone marrow, PB=peripheral blood. Bars represent means±s.e.m. (MYC n=4, DT-FF n=5, DT-A1 n=6 biological replicates, three independent experiments). ANOVA followed by Bonferroni post hoc test. *P⩽0.05, **P⩽0.01, ***P⩽0.001.