Figure 6
From: MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis
Transgenic overexpression of BFL-1 rescues premalignant B cells from the effects of A1 knockdown. Flow cytometric analysis of single-cell suspensions from blood, bone marrow, lymph nodes and spleen. (a) Representative dot-plots showing percentages of sIgM+ and sIgM− B cells in major lymphatic organs. Cells from premalignant mice of the indicated genotypes were stained with antibodies recognizing B220 or IgM, and analyzed further based on Venus-reporter expression (IgM− cells representing pro/pre-B cells and IgM+ cells representing immature/mature B cells). The percentage of cells falling within each gate is shown. Data shown in (a) are quantified in (b). Bars represent means±s.e.m. (MYC n=8, DT-A1 n=13, TT-A1 n=4 biological replicates, six independent experiments). ANOVA followed by Bonferroni post hoc test. *P⩽0.05, **P⩽0.01, ***P⩽0.001. LN=lymph nodes, BM=bone marrow. NS=not significant.
