Figure 5
Tumour formation and metastasis in mice requires Lysine-270 and lysine-277 of Hsp90α. (A) Results of a representative experiment showing tumour formation of injected (5 × 106) native MDA-MB-231 (panels a and b), KO-α-#1 cells alone (panel c) or KO-α-#1 cells supplemented with wild-type human recombinant Hsp90α (panels d and e), or with human recombinant Hsp90α-G/T mutant (panel f) or wild-type human recombinant Hsp90β (panel g) or with human recombinant Hsp90β-K/K mutant (panel h) protein to the mammary fat pad of nude mice (n=5 per group). The detected tumour formation was indicated by dotted circles and excised tumours measured for their Tumour Volume (TV, mm3). (B) Histological (H&E) analyses of the tumour, mammary fat pad tissue and the lung cryosections from non-tumour and tumour-containing mice as indicated. This experiment was repeated three times (n=4 or 5 per group). hr, human recombinant; T (red), tumour; N (blue), normal tissue; 5/5, five out of the five mice; Data are represented as mean±s.e.m. P<0.05.
