Figure 6
Development of secreted Hsp90α-neutralizing monoclonal antibodies against the dual lysine region of human Hsp90α. (a) A schematic illustration of the antigen (F-5) location in reference to the dual lysine residues in full-length Hsp90α. (b) IgG was purified by protein-G Sepharose affinity chromatography from two hybridoma clones, 1G6-D7 and 5C4-D4, and analysed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) with coomassie brilliant blue staining. Regular mouse IgG was used as a control. (c) 1G6-D7 (IgG1κ) and 5C4-D4 (IgG2aκ) are mapped to bind to TKPIWTRNP and VKHFSVEGQ, respectively, within the antigen.
