Figure 2

ZNF750 is under-expressed in squamous cell carcinomas. (a) ZNF750 mRNA expression across normal tissues examined from GEO (series GSE7307). Data show mean±s.d. (b) ZNF750 protein expression across normal tissues from Human Protein Atlas (see URL). IHC was performed using anti-ZNF750 antibody (HPA021573, Sigma-Aldrich, St Louis, MO, USA). (c) Representative IHC photos (left panel) and IHC scores (right panel) of ZNF750 protein expression in CSCC and HNSCC from Human Protein Atlas. IHC was performed using anti-ZNF750 antibody (HPA023012, Sigma-Aldrich). (d) In-house IHC assay of ZNF750 expression in CSCC, HNSCC and LSCC samples. Normal tissues are from cervical tissues (× 10 magnification). (e) Low ZNF750 expression (mRNA expression z-Scores (RNA Seq V2 RSEM)>mean+1.8 SD or 2.0 SD) was associated with poor disease-free survival of HNSCC and LSCC patients in the TCGA cohorts.