Figure 4

Tumour-suppressive properties of ZNF750 in SCCs. (a–c) Short-term proliferation assay (MTT) (a), foci formation assay (b) and the quantification of SCC cell growth (c) either with ectopic expression of GFP control (CTL), wildtype or C2H2 mutant ZNF750. Data show mean±s.d. N=3. *P<0.05. (d) UMSCC1 cells expressing indicated vectors (upper panel) were injected subcutaneously on the upper flanks of NOD-SCID mice, and tumour weights were measured. Data represent mean±s.d. *P<0.05. (e) Representative samples of Ki67 and IVL expression detected by IHC in dissected xenograft tumours. (f–g) SCC cells expressing indicated vectors were subjected to migration assay (f) and adhesion assay (g). Data show mean±s.d. N=3 (f) and 4 (g). *P<0.05. (h-i), Dead cells (trypan-blue positive) (h) and cleaved-PARP (i) in SCC cells expressing indicated vectors upon anoikis challenge. Data show mean±s.d. N=4 (h). *P<0.05.