Figure 6
From: ZNF750 is a lineage-specific tumour suppressor in squamous cell carcinoma
ZNF750 regulates epidermal differentiation in a variety of SCC cells. (a) qRT-PCR analysis of differentiation related genes and (b) basal cell marker K14 gene in SCC cells expressing indicated vectors. Data show mean±s.d. N=3. *P<0.05. (c) Western blot analysis of IVL and K14 levels in SCC cells expressing indicated vectors. (d) qRT-PCR analysis of differentiation related genes in SCC cells treated with either scrambled siRNAs (siCTL) or ZNF750 siRNAs (siZNF750). Data show mean±s.d. N=3. *P<0.05. (e) Western blot analysis of IVL and K14 levels in SCC cells treated with indicated siRNA. (f) Co-expression analysis of ZNF750 mRNA and epidermal differentiation gene mRNA based on TCGA dataset. r; spearman score. (g) Summary of correlation coefficient between the mRNA levels of ZNF750 and indicated differentiation genes across different tumour types from TCGA. (h) Western blot analysis of ZNF750 protein levels in SCC cells under proliferative condition (cells<50% confluent) (P) or differentiation condition (super confluent) (D) in vitro. (i) qRT-PCR analysis of differentiation related genes including KLF4 in non-SCC cell line HaCaT treated with scrambled siRNAs (siCTL) or ZNF750 siRNAs (siZNF750). Data show mean±s.d. N=3. *P<0.05.
