Figure 8
From: ZNF750 is a lineage-specific tumour suppressor in squamous cell carcinoma
ZNF750 represses LAMC2 transactivation. (a) qRT-PCR analysis of LAMC2 mRNA levels in SCC cells expressing indicated vectors. Data show mean±s.d. N=3. *P<0.05. (b) Western blot analysis of LAMC2 levels in SCC cells expressing indicated vectors. (c) ZNF750 motifs present in R2, R3 and R4 DNA fragments 5’ upstream of LAMC2 (R1: nucleotide (nt) −3701/−3528; R2: nt −2506/−2273; R3: nt −737/−570; R4: nt −346/−182) (lower). (d) Chip-PCR analysis of DNA fragments pulled down by an anti-HA antibody. IgG was used as a negative control. Representative data are shown. N=3. (e) 293 T cells transfected with the reporter constructs pGL3-(R2), renilla, and either GFP or wildtype ZNF750. Renilla and luciferase activity was analysed. Data show mean±s.d. N=3. *P<0.05. (f) Western blot analysis of LAMC2 and ZNF750 levels in SCC cells in indicated conditions. (g) SCC cells in indicated conditions were subjected to migration assay. Data show mean±s.d. N=3. *P<0.05.
