Figure 2
MCF7-Y537S cells grow in an estrogen-independent manner. (a) MCF7 (WT) and Y537S cells were grown in the absence or presence of 10 nM estrogen over 12 days. (b, c) The cells were grown in the absence of estrogen (vehicle) or in the presence of 10 nM estrogen, together with 0, 1, 10, 100 or 1000 nM 4-hydroxytamoxifen (OHT; b) or faslodex (FAS; c). Mean growth at day 12 ±s.e.m. is shown relative to day 0 (n=6). (d) MCF7 and MCF7-Y537S cells, cultured in DMEM+10%FCS, were treated with OHT or FAS at the concentrations shown (nM). The graph shows mean growth at day 12±s.e.m. (n=6).
