Figure 1
From: Wwox–Brca1 interaction: role in DNA repair pathway choice
Human and mouse Wwox-deficient cells exhibit increased survival to DSBs. Wwox-deficient cell lines are represented in red or orange, Wwox-sufficient cell lines are represented in blue or green. (a) Graph depicting % survival of MEF cell lines, Wwox knockout (KO3, KO5) and wild type (WT4, WT7), following IR treatment. (b) Graph depicting % survival in the transformed breast epithelial MCF10 cells and Wwox stably silenced MCF10A clones, shWWOXA and shWWOXB, following increasing IR doses. (c) Graph depicting % survival in doxycycline-inducible MDA MB-231 cells following IR treatment. (d) Graph depicting % survival in MCF10A cells and shWWOXA and shWWOXB clones following bleomycin treatment. (e) Graph depicting % survival of doxycycline-inducible KO3 clone, 2A2, with and without doxycycline after IR treatment. (f) Graph depicting % survival of doxycycline-inducible KO5 clone, 2F2, with and without doxycycline after various IR doses. (a–f) Embedded western blots (WBs) demonstrate Wwox expression in the cell lines. Experiments were performed in triplicate. Error bars denote 1 s.d.; lower standard error bars are abbreviated due to log scale.
