Figure 4

Cell cycle characterization in Wwox MEFs and 231/Wwox cells after IR. (a) Stacked column graph depicting cell cycle distribution of 231/Wwox cells with and without doxycycline at designated time points after 10 Gy IR. Bottom panel depicts corresponding protein expression with darker shades of blue indicating increasing intensity. (b) Representative images of flow cytometric DNA content analysis in 231/Wwox cells with and without doxycycline at 0, 1, 10 and 24 h after 10 Gy IR. (c) Stacked column graph depicting cell cycle distribution in wild-type and knockout MEFs at designated time points after 10 Gy IR. Bottom panel depicts corresponding protein expression with darker shades of blue indicating increasing intensity of checkpoint proteins. (d) Representative flow cytometric analysis of DNA content in WT7, KO3 and KO5 at 0, 1, 10 and 24 h after 10 Gy IR. (a, b) The 231/Wwox cells were untreated or induced for Wwox expression by treatment with 1 μg/ml doxycycline for 24 h before IR treatment and harvesting for flow cytometry, as described.⁴¹