Figure 6

Metformin-induced H19 repression and alteration of gene methylation are recapitulated in endometrial cancer patients treated with antidiabetic doses of metformin. (a) Results of western blot analysis of AMPK phosphorylation in five paired specimens from before (Pre) and after (Post) metformin treatment. P-value is shown on top of the bar graph. (b) Results of quantification of immunostaining of Ki-67 in five paired endometrial cancer tissues. (c) Results of reverse transcriptase–quantitative PCR analysis of H19 RNA levels in five paired endometrial cancer tissues. (d) Results of QMSP of methylation of the indicated genes in four paired endometrial cancer tissues. P-values are shown on top of each bar graph. (e) A model for metformin-induced, H19-mediated alteration of gene methylation in cancer cells.