Figure 4
From: TGF-β upregulates the translation of USP15 via the PI3K/AKT pathway to promote p53 stability
Competitive recognition of both MDM2 and p53 by USP15. GST-p53 (0.1 μg) was incubated with His6-MDM2 (0.6 μg) to form the complex, followed by addition of GST-USP15 or GST-USP15-C269A as the indicated amount. All components were subjected to precipitation by the Ni2+-Sepharose. The precipitated products were analyzed by western blotting and probed for p53, USP15 and His6 tag. (a) USP15 disrupts the MDM2/p53 complex. (b) Replacement of the active site, Cys269, by Ala does not affect the competitive recognition of both MDM2 and p53 by USP15. Input: GST-p53 (0.033 μg); GST-USP15 (0.16 μg); GST-USP15-C269A (0.16 μg).
