Figure 1
From: ER stress protein AGR2 precedes and is involved in the regulation of pancreatic cancer initiation
AGR2 expression in human pancreas. (a) Immunohistochemical analysis of AGR2 expression in chronic pancreatitis (CP) and PDAC peritumoural tissues. AGR2 protein expression was absent in most of the pancreas (top left) but induced in early PanINs (top right) and tubular complexes, CT (central left), as well as focally induced in phenotypically normal acinar (central right), centroacinar cells (bottom left) and duct/terminal duct (bottom right). (b) AGR2 expression in representative peritumoural area. Low magnification of a pancreas tissue (top left) showing area with no AGR2 expression (top right), AGR2 induction in acinar cells (central panels) and AGR2-expressing putative pre-neoplastic lesions, TC (bottom left) and PanIN (bottom right). (c) AGR2 expression in representative CP tissue. Low magnification of a pancreas (top left) showing area with no AGR2 expression (top right), moderate AGR2 induction in small and terminal ducts (central left) and strong AGR2 expression in TC and PanINs (central right and bottom panels). Scale bars, 50 μm. (d) FACS sorted autofluorescent (Fluo+) and non-autofluorescent (Fluo-) populations of two different PDX-derived primary cell lines (A6L and 185, top panels) were analysed for AGR2 expression by Western blot (bottom left). Densitometry analysis is shown on the right panel.
