Figure 2
From: ER stress protein AGR2 precedes and is involved in the regulation of pancreatic cancer initiation
AGR2 loss impairs development of pancreatic pre-neoplastic lesions. (a) The absence of AGR2 expression in control LSL-KrasG12D/+ (K) mice pancreas. (b) AGR2 expression in LSL-KrasG12D/+;Pdx1-Cre (KC) mouse model. AGR2 expression in acinar cells in 2 weeks-old KC mice (top left) and in tubular complexes (top right), low-grade and high-grade PanINs (left and right bottom panels, respectively) in 4–12 weeks old mice pancreas. Scale bars, 50 μm. (c) Genetic make up to activate oncogenic Kras in the pancreas using Cre-loxP recombination system and subsequent crossing with the Agr2 knock-out mouse model. (d) Representative images of H&E staining of 1 month-old KC and KCA mice pancreas. (e) Cytokeratin 19 (CK19) staining of tubular complexes (left) and early PanINs (central and right panels) in 1 month-old KC mice. Scale bars, 50 μm. (f) Quantification of the numbers of pre-neoplastic lesions and their foci in KC (n=13) and KCA (n=18) mice groups. ***P<0.001.
