Figure 3
From: ER stress protein AGR2 precedes and is involved in the regulation of pancreatic cancer initiation
AGR2 is induced by ER stress in pancreatic cells and regulates ER homoeostasis. (a) Real-time PCR analysis showed XBP1s and AGR2 gene overexpression in HPDE (KRAS wt), T3M4 (KRASH61Q), and FA6 and CFPAC1 (both KRASG12D) pancreatic cell lines treated with 5 μM of tunicamycin (Tm) for 2, 6 or 24 h. S16 was used as a control gene. Western blot analysis (right panels) showed AGR2 protein accumulation after 24 h tunicamycin-treatment; tubulin protein was used as a loading control. (b) 72 h after siRNA silencing of AGR2 in CFPAC1 and FA6 cells (top), overexpression of XBP1s gene was observed by real-time PCR (bottom panels). *P<0.05; **P<0.01.
