Figure 5
From: ER stress protein AGR2 precedes and is involved in the regulation of pancreatic cancer initiation
ER-stressed pancreatic cells adopt a pro-inflammatory phenotype. (a) ER stress induction analysis in PS1 human pancreatic stellate cells. PS1 cells were treated with 1 μM tunicamycin or vehicle for the indicated times (top panels). XBP1s and IL6 gene expression was analysed by real-time PCR and IL6 protein levels by ELISA analysis of culture supernatants (right panel). PS1 cells were treated with conditioned media from ER-stressed or control pancreatic cancer cells and XBP1s and IL6 gene expression analysed by real-time PCR (bottom panels). (b) ER stress induction analysis in human normal pancreatic HPDE cells after treatment with 5 μM Tm (top panels), with conditioned media from ER-stressed or control pancreatic cancer cells (central panels), or from ER-stressed or control PS1 pancreatic stellate cells (bottom panels). XBP1s, AGR2 and IL6 gene expression was analysed by real-time PCR. Ctrl, control; CM, ER stress-conditioned medium; PC, pancreatic cancer cells; Tm, Tunicamycin. *P<0.05; **P<0.01; ***P<0.001.
