Figure 3

NRP1 expression is dynamically regulated during the adaptive response to ATTs and progression to mCRPC. (a) Microarray analysis of NRP1 expression in LNCaP xenografts harvested from non-castrated mice (intact) and during progression to CR after castration of host nude mice. (b) Western blot of NRP1 in parental LNCaP cells and their androgen-independent variant, LNCaP-AI. (c) NRP1 mRNA expression in hormone naive primary PCa biopsies compared with hormone refractory samples from data set GDS1390.24 (d) NRP1 mRNA levels in benign, localized PCa and mCRPC samples from Grasso et al.11 Data extracted from GSE35988. (e) Scatterplot showing log2 RPKM normalized NRP1 RNA-seq read counts from individual mCRPC samples (n=118) from the Stand Up To Cancer (SU2C)/Prostate Cancer Foundation (PCF) Dream Team cohort.25 Data obtained from cBioPortal.52, 53 (d, e) Error bars represent s.e.m. ****P <0.0001.