Figure 2
From: Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression
Pin1 silencing influences the TALL-1 cells invasiveness by regulating N3IC protein expression. (a) Western blots against Pin1 show the efficiency of Pin1 silencing in TALL-1 cell line (siPin1). (b) TALL-1 cell line silenced or not for Pin1 was used in invasion Matrigel assay: relative percentage of invasiveness is shown with respect to the negative control, siCTR (left panel). RT–PCRs show downmodulation of MMP9 mRNA expression in Pin1-silenced cells (siPin1) with respect to the control cells (siCTR) (right panel). (c) Western blots against activated-N3IC protein (N3IC-act) and Pin1 show the efficiency of the Notch3 receptor block and Pin1 silencing, respectively (lower panels). Optical densitometry (OD) of the activated-N3IC protein expression (upper panel). (d) RT–PCRs show downmodulation of MMP9 mRNA expression in Notch3-blocked Pin1-silenced cells (siPin1+FCNotch3) with respect to both Notch3-blocked or Pin1-silenced controls alone. In both panels (a) and (c), western blot against the anti-β-actin was used as a loading control. All the results shown in the figure are expressed as the means average deviations of three separate experiments, each in triplicate, and P-values were calculated using Student’s T-test (i.e., ns, not significant P>0.05; *P⩽0.05; **P⩽0.01). WCEs, whole-cell extracts.
