Figure 2
From: TGFβ engages MEK/ERK to differentially regulate benign and malignant pancreas cell function
TGFBR-deficient mice display loss of cell cycle control despite reduced ERK activation. (a and b) Tissue sections from KRAS, KT2 and KT1 mice were stained for p21, indicating reduced expression in both TGFBR-deficient cohorts. (c–e) Tissues were stained for the p21 targets CDK2 and Cyclin E, indicating overexpression in KT2 and KT1 mice compared with KRAS controls. (f–h) We next assessed pRB and PCNA, surrogate markers of proliferation and found strong staining for both in neoplastic tissues of KT2 and KT1 mice compared with modest staining in KRAS controls. (i–k) Tissue sections from wild type (WT), Tgfbr2DN, and Tgfbr1+/− mice were similarly stained for CDK2 and Cyclin E, both of which were similarly upregulated in Tgfbr2DN and Tgfbr1+/−. (*P<0.05. N=4 mice per group unless otherwise specified).
