Figure 5
From: KLF6 depletion promotes NF-κB signaling in glioblastoma
Wild-type KLF6 induces neural-like differentiation in LN229 cells. (a) Microphotographs of green fluorescent protein-positive LN229 cells expressing empty vector (EV), KLF6-wt or KLF6-sv1 after lentiviral infection. (b) qRT-PCR analysis of neuronal (TUBB3 and NEFM), astrocytic (GFAP) and stem cell (NESTIN) markers in LN229 cells expressing EV, KLF6-wt or KLF6-sv1. (c) Immunoblotting of TUBB3 in LN229 cells expressing EV, KLF6-wt or KLF6-sv1. (d) Corresponding immunofluorescence staining for neuronal marker TUBB3 in LN229 cells expressing EV, KLF6-wt or KLF6-sv1. Nuclei were stained with DAPI. The scale bar represents 100 μm (a) or 50 μm (d). Relative gene expressions are normalized to EV, a representative qRT-PCR of three independent experiments is shown (b). Error bars represent mean±s.d.
