Figure 7
Neutralization of GM-CSF and IL-6 reverses aggravated orthotopic HCC progression and MDSC accumulation in Rarres2−/− mice. (a) Kaplan–Meier plot of survival and representative images of liver tumors and the liver weight. of WT mice and Rarres2−/− mice received isotype antibodies or neutralizing antibodies for GM-CSF and IL-6 in an orthotopic HCC model. n=16. (b) Representative images of hematoxylin and eosin (H&E)-stained lung sections and numbers of metastatic nodules counted in a 100X high-power field (HPF). (c) The percentages of MDSCs and macrophages in tumor tissues and MDSCs in blood and spleens, as well as IFN-γ+CD8+ T cells and IFN-γ+CD4+ T cells in tumor tissues. (d) Representative images of IHC staining of CD31. (e) Quantitative reverse transcriptase (qRT)–PCR analysis of the expression of proangiogneic genes in tumor tissues. Original magnification, × 200; scale bar, 50 μm in b and d. Columns and error bars represent mean±s.e.m. (n=7 per group). Similar results were obtained from two independent experiments. **P<0.01, ***P<0.001. (f) Schematic diagram of the cellular and molecular mechanism by which chemerin suppresses HCC progression.
