Figure 4 | Oncogene

Figure 4

From: Caspase-8 tyrosine-380 phosphorylation inhibits CD95 DISC function by preventing procaspase-8 maturation and cycling within the complex

Figure 4

Y380 phosphorylation inhibits procaspase-8 inter-dimer processing at the CD95 DISC. (a) CD95-IcD pull-downs from Jurkat caspase-8 null lysates using active site WT Casp-8b (WT, Y380E or Y380F) or C360A mutants of recombinant Casp-8a, either singly or in a 1:1 ratio. Beads and supernatant were analysed for recombinant caspase-8, FADD and lysates for active caspase-3 (Asp175) and IETDase bead activity measured. (b) Graphical representation of IETDase values from (a). Normalized against wild-type caspase-8b alone. Data are mean±s.e.m., n=3, *P<0.05.

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