Figure 5 | Oncogene

Figure 5

From: Caspase-8 tyrosine-380 phosphorylation inhibits CD95 DISC function by preventing procaspase-8 maturation and cycling within the complex

Figure 5

Caspase-8 phosphorylation inhibits apoptosis by impeding native CD95 DISC function. (a) Apoptosis in SH-SY5Y cell clones stably expressing WT or mutant procaspase-8b after 4 h exposure to anti-CD95 (αCD95; 400 ng/ml) (mean+s.e.m., n=3). (b) Immunoblots of whole-cell lysates following anti α-CD95 treatment using antibodies raised against caspase-8, FADD, BID and caspase-3. (c) CD95 DISC Pulls from SH-SY5Y cells expressing phosphorylation site Casp-8b WT, Y380E or Y380F mutants. Beads and supernatant were immunoblotted for FADD, caspase-8 cleavage fragments and active caspase-3. (d) IETDase activity of CD95 DISC Pulls from SH-SY5Y cells expressing phosphorylation site Casp-8b WT, Y380E or Y380F mutants. Values are mean+s.e.m., n=3, *P<0.05.

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