Figure 4
From: Novel MYC-driven medulloblastoma models from multiple embryonic cerebellar cells
MBG3 from cerebellar inhibitory interneuron progenitors. (a) Kaplan–Meier survival curve for mice developing Atoh1-MYC (n=3/3, red curve), Gad2-MYC (n=4/11, blue curve) and Ptf1a-MYC (n=3/7, green curve) MBs. Atoh1-MYC tumors developed significantly faster than Gad2-MYC MBs (P<0.0001, a two-tailed P-value). (b–d) Serial bioluminescence images of representative (b) glutamatergic (Atoh1-Cre) and (c and d) GABAergic (Gad2-IRES-Cre (c) and Ptf1a-Cre (d)) mice subjected to EP with LRL-Myc-Luc, Trp53DN-copGFP and T2TP. (e–j) Tumors stained with H&E (e, g and i) and an anti-Npr3 antibody (f, h and j). Scale bar=25 μm. (k and l) Distribution of GFP+ cells in electroporated cerebella of P2 Atoh1-Cre (k) and P3 Gad2-IRES-Cre (l) animals. (sagittal sections, scale bar=100 μm). (m) Heat maps of subgroup-specific genes differentially expressed across EP-based MBG3 models.
