Figure 4
CECR1-mediated macrophage paracrine activation of angiogenesis is partially regulated via PDGFB. (a and b) Low- and high-magnification fluorescent images of neo-vessel formation by HUVECs (GFP-marked) and human-derived pericytes (dsRed-marked) without THP1 macrophage stimulation, and with stimulation of non-treated (control), and sisham- or siPDGFB-treated THP1 macrophages, without or with rhCECR1 rescue. (c–e) Quantified results of the co-culture experiment. Number of junctions, tubules and total tubule length data are shown. *P<0.05 (MФ (-) versus MФ-control, MФ-sisham versus MФ-siPDGFB, MФ-sisham CECR1 0 nm versus 50 nm, MФ-siPDGFB CECR1 0 nm versus CECR1 50 nm); **P<0.01 (MФ-sisham CECR1 0 nm versus 50 nm in number of tubules), ***P<0.005 (MФ-sisham CECR1 0 nm versus 50 nm in total tubule length). Scale bar, 100 μm. N>10 co-cultures in total.
