Figure 6

Effects of miR16 on colony formation ability of glioblastoma cells and differentiation of GSCs. (a) The ability of colony formation (number and size) in glioblastoma cells was evaluated by soft-agar assay (magnification, × 100). Comparison of the difference of this ability between groups of miR16 mimic and miR16 inhibitor or NC was performed. (b) The ability of sphere formation (number) in glioblastoma cells was examined by sphere-forming assay. The number of sphere between groups of miR16 mimic and miR16 inhibitor or NC was compared. The data are present as the mean±s.d. from three independent experiments with triplicate in each condition. (c) The differentiation of U87-GSCs was verified by the cell surface markers that can be identified by immunofluorescence (middle, next right, magnification, × 200) and flow cytometry (right). The suspension ball of U87-GSC (CD133+/GFAP−) (left) was gradually transformed into U87 cells (CD133−/GFAP+) (middle, next to left) in the medium with serum in 5 days.