Figure 2 | Oncogene

Figure 2

From: MicroRNA-20a-mediated loss of autophagy contributes to breast tumorigenesis by promoting genomic damage and instability

Figure 2

miR-20a targets several autophagy related genes. (a) Relative mRNA levels (normalized by GAPDH) of BECN1, SQSTM1, ATG16L1 in MCF7 or (c) MDA-MB-231 cells overexpressing NC or miR-20a were analyzed by qPCR. (b) MCF7 or (d) MDA-MB-231 cells transfected with NC, miR-20a, LNA-NC or LNA-20a were either untreated or treated with EBSS for 4 h. The protein levels of ATG16L1, BECN1 and SQSTM1 were analyzed by immunoblotting. (e) Schematic illustration of the predicted miR-20a pairing sites in BECN1, SQSTM1 and ATG16L1 3′UTRs. Luciferase reporters containing wild type (WT) or mutated (MUT) BECN1, SQSTM1 or ATG16L1 3′UTR fragments were co-transfected with NC or miR-20a into MCF7 cells. Relative luciferase activity was normalized to NC (**P<0.01).

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