Figure 1

Rho target genes are repressed by EwS-FLI1 in EwS. (a) Gene set enrichment analysis (GSEA) plots showing the differential analysis of 117 EwS tumours (GSE34620)¹⁷ versus mesenchymal stem cells (MSCs; GSE31215).¹⁸ Genes involved in the response to serum, RhoA or are involved in migration are repressed in the EwS tumours versus MSCs. (b) Heatmap showing a manually curated set of Rho/SRF target genes under EWS-FLI1-high or -low conditions in six different EwS cell lines: left panel: WE68, TC252, SK-N-MC, STA-ET-7.2, STA-ET-1 (data from GSE14543),⁶ and right panel: A673/TR/shEF, this study: two replicates. Expression values are scaled row-wise.