Figure 3

CAIX regulates migration of breast cancer cells. (a) Images of wound-induced cell migration by the indicated MDA-MB-231 cells cultured in normoxia. Scale bar, 300 μm. Dashed lines demarcate the wound boundary at t=0 h and yellow shading indicates the cell-free area. (b) Quantification of migration by the cells described in a. ***P<0.001. (c) Images of wound-induced migration of MDA-MB-231 CAIX-BirA* cells cultured in normoxia and treated with increasing concentrations of U-104. Scale bar, 300 μm. Wound boundaries and cell-free area are demarcated as described in a. (d) Quantification of migration by the cells described in c. *P<0.05, ***P<0.001. Data in panels (b) and (d) show the mean±s.e.m. of technical replicates (n=16) and are representative of two independent experiments. (e) Wound-induced migration of WT MDA-MB-231 cells cultured in normoxia (black bars) or hypoxia (gray bars) and treated with U-104. Data show the mean±s.e.m. of technical replicates (n=12) and are representative of three independent experiments. **P<0.01, ***P<0.001. Statistical analysis was performed using Student’s t-test (b) or ANOVA (d,e).