Figure 6 | Oncogene

Figure 6

From: Elevation of adenylate energy charge by angiopoietin-like 4 enhances epithelial–mesenchymal transition by inducing 14-3-3γ expression

Figure 6

The ANGPTL4/14-3-3γ axis coordinates energy demand during EMT. (a, e) Immunoblot analysis of 14-3-3γ expression after TSC2S939 (a) or Snai1 (e) immunoprecipitation in MKN74Ctrl and MKN74ΔANGPTL4 cells. (b–d and f, h–i) Proximity ligation assay (PLA) quantification of 14-3-3γ/TSC2S939 and 14-3-3γ/Snai1 interactions in MKN74CTRL and MKN74ΔANGPTL4 cells (b, f), xenograft tumors (c, h), and tumor biopsies from patients (d, i) after the indicated treatments or with indicated disease stages. (g) Immunodetection of the indicated proteins in hypoxic-induced MKN74CTRL and MKN74ΔANGPTL4 cells at 0 and 48 h. (j) Representative ChIP results using anti-Snai1 (upper panel) followed by re-ChIP with anti-14-3-3γ (lower panel) in MKN74CTRL and MKN74ΔANGPTL4 cells under normoxic or hypoxic conditions. Preimmune serum (IgG) served as control. (k) Schematic diagram depicting the coordination of metabolic reprogramming by the ANGPTL4/14-3-3 axis during EMT. Data are represented as mean±s.d. from at least three independent experiments. *P<0.05, **P<0.01. Loading controls for the immunoblot analyses were from the same sample.

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