Figure 1 | Oncogene

Figure 1

From: Identification of translationally controlled tumor protein in promotion of DNA homologous recombination repair in cancer cells by affinity proteomics

Figure 1

Proteomic identification of TCTP interactome by immunoprecipitation (IP). (a) Schematic of the workflow of profiling of TCTP-interacting proteins by IP combined with LC-MS/MS. Cell lysates were prepared from HeLa cells stably expressing 3 × Flag-TCTP or control cells transformed with the empty vector. The M2 antibody-coupled magnetic beads were used for IP. The resulting bound proteins were resolved on a 15% SDS–PAGE gel, the gel was stained with Coomassie blue and cut into slices. Peptides were extracted from in-gel trypsin digestion and subjected to LC-MS/MS. (b) Western blot (WB) verification of 3 × Flag-TCTP overexpression in HeLa cells. M2 antibody was used at 1:1000 of dilution, and GAPDH was as a loading control. (c) The efficiency of anti-Flag antibody-mediated TCTP IP. M2 antibody was used for both IP and WB. Bound proteins were resolved on 15% SDS–PAGE gels. IgG-L and IgG-H are the light and heavy chains of M2 antibody.

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