Figure 4 | Oncogene

Figure 4

From: Using a novel computational drug-repositioning approach (DrugPredict) to rapidly identify potent drug candidates for cancer treatment

Figure 4

Indomethacin exerts additive effect with cisplatin in ovarian tumor cells. (a) 48 h MTT analysis of OV81.2 and OV81.2-CP10 cells treated with increasing doses of cisplatin and cisplatin plus Indomethacin. (b) Isobologram analysis of the MTT data of Indomethacin cisplatin combination treatment in OV81.2 and OV81.2-CP10 cells showing additive effect of the combination of the two drugs. (c) Dose–response clonogenics assay on day 7 showing effect of Indomethacin and cisplatin combination on cell survival in OV81.2 and OV81.2-CP10. (d) 48 h Flow cytometry analysis of Annexin- V PI staining showing increased cell death upon Indomethacin and cisplatin combination treatment with the corresponding isobologram doses in OV81.2, OV81.2-CP10 and CP70. (e) Western blots showing elevated cleaved-PARP and γ-H2AX protein levels upon 24 h Indomethacin and cisplatin combination treatment with the corresponding isobologram doses in OV81.2-CP10 and CP70 (**P<0.005 and ***P<0.0005).

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