Figure 4
Loss E2f7/8 in xenografted MEF tumors in zebrafish leads to increased intratumoral blood vessel branching. Experimental setup similar as described in Figure 3a. (a) Representative image and quantification (n=10) of hyperbranching in control and E2f7/8 DKO tumors injected in Tg(fli1a:gfp) (black color; endothelial cells). (b) Indicated mRNA levels measured in control or E2f7/8 DKO MEFs cultured under standard conditions using qPCR. (c) Indicated mRNA levels measured in zebrafish xenografts of control (eight tumors) or E2f7/8 DKO MEFs (five tumors). All quantified data present the average±s.d. compared with the indicated controls. ***P<0.05; ns, not significant.
