Figure 5
From: A slow-cycling subpopulation of melanoma cells with highly invasive properties
SerpinE2 expression is increased in malignant cells and correlates with tumor progression. (a) Western blot analysis of SerpinE2 secretion by melanoma cells (n=21) and human melanocytes (n=5) into culture supernatants. Recombinant SerpinE2 protein (500 ng) was used as a positive control and Ponceau’s staining is shown for loading control. Graph shows quantification (right panel). (b) SerpinE2 staining of 3D skin reconstructs with melanocytes (left) and melanoma cells (right). (c) GEM_1375 data set analysis of SerpinE2 mRNA expression in melanomas, non-malignant nevi and normal skin. (d) Examples of immune histochemistry at two different magnifications of: normal skin, benign nevi, in situ, vertical growth phase (VGP) and lymph node metastatic melanomas (n=15 for each group). SerpinE2 protein expression is indicated by purple staining. Arrows indicate the dotted SerpinE2 expression pattern found in benign nevi only. (e) Intensity score of SerpinE2 expression in normal skin, benign nevi and malignant melanomas (radial growth phase (RGP), VGP and metastatic). Bars represent mean±SEM of tissue sections of 15 specimens from each group; P-value after t-student test are given.
