Figure 2
ZEB1-regulated miRNA clusters block VM. (a) Quantitative reverse transcription–PCR (qRT–PCR) for ZEB1 in MDA-MB-231 cells transfected with siNT control or siZEB1. Error bars represent s.d. of 3 independent experiments. (b) Western blot analysis (left) and quantitation (right) of ZEB1 in control vs knockdown cells. ACTB is loading control. Error bars represent s.d. from 3 independent experiments. (c) Phase images of VM assay in control or ZEB1 knockdown cells. Scale bars=1000 μm. (d) Quantification of total network length over 10 independent experiments. Error bars represent s.d., t-test was performed, ***P<0.001. (e) qRT–PCR for miRNAs in cells transfected for 72 h with siNT or siZEB1. Error bars represent s.d. of 3 independent experiments. T-tests were performed, **P<0.01; ***P<0.001. (f) Phase images of VM assay with NEG or miRNA mimic transfected cells. Scale bars=1000 μm. (g) Quantification of total network length. Error bars represent s.d. for 3 independent experiments. T-tests were performed, *P<0.05. (h) Phase images of MDA-MB-231 cells transfected with miRNAs as shown. Scale bars=100 μm. (i) qRT–PCR following expression of NEG control or miRNA mimics. Error bars represent s.d. of 3 independent experiments. T-tests were performed for each condition vs NEG control, ***P<0.001. (j) Western blot analysis in cells transfected with NEG mimic or miRNA mimics. ACTB is loading control. (k) Quantitation of proteins normalized to ACTB in 3 independent experiments. Error bars represent s.d. T-tests were performed for each protein in cells transfected with each miRNA cluster vs NEG control, *P<0.05, ***P<0.001.
