Figure 2
Lipid biosynthesis is the major target of AR in VCaP and VCS2 cells. (a, b) Integrated analyses of AR ChIP-seq and AR-regulated gene expression in VCaP and VCS2 cells (10 nM DHT for 24 h). Gene Ontology analysis of direct AR-activated genes in (a) VCaP cells and (b) VCS2 cells. This analysis is based on AR-binding sites within 20 kb of transcriptional start sites and two-fold cutoff for change of expression. (c) The list of identified AR-activated genes mediating variety of lipid synthetic pathways. (d) The list of VCS2-specific AR-regulated genes with AR-binding. (e) AR ChIP-seq signal of a panel of identified AR-binding sites within 7 normal prostate samples and 13 PCa samples (database: GSE70079).
