Figure 3
AR-mediated lipid biosynthesis is restored in CRPC. (a) qRT-PCT analysis for expression of indicated genes in VCS2, VCaP, LNCaP cells treated with vehicle or DHT (10 nM) for 24 h. (b) ChIP-qPCR analysis for AR recruitment at indicated gene loci in VCaP and LNCaP cells treated by DHT alone (4 h) versus DHT plus enzalutamide (pretreat 4 h). (c) mRNA from tissue biopses of VCaP xenografts (n=6) at pre-castration (AD) and at relapse (CRPC) were extracted and subjected to qRT-PCR analysis. Gene expression is normalized to its level at pre-castration. (d) Box plot for expression of indicated genes in hormone-dependent primary PCa versus CRPC bone metastases (database: GSE32269). (e) Kaplan–Meier plotting was performed for the 32-gene subset of lipid synthesis genes on the TCGA, Taylor and Glinsky PCa cohorts. Blue curve represents bottom 50% patients with low expression and red curve represents top 50% patients with high expression.
