Figure 4
The expression of AR-V7 contributes to the reactivation of lipid synthesis in CRPC cells. (a) qRT-PCR analysis for AR-V7 in VCS2 and VCaP cells. (b) ChIP-qPCR analysis for AR-V7 recruitment using AR-V7 specific antibody at indicated gene loci in VCaP cells treated by DHT for 4hr. (c) qRT-PCR analysis for indicated genes in CWR22-RV1 cells treated with DHT for 24 h. (d) ChIP-qPCR analysis for AR-V7 recruitment at indicated gene loci in CWR22-RV1 cells. (e) ChIP-qPCR analysis for total AR and AR-V7 recruitment at indicated gene loci in CWR22-RV1 cells treated by DHT alone (4hr) versus DHT plus enzalutamide (pretreat 4 h). (f–h) CWR22-RV1 cells were transfected with siNTC, siCE3, or siE7 for 2 days (d) in hormone-depleted medium and then treated with (f) 0–10 nM DHT or (g, h) 0–1 nM DHT for 24 h, followed by (f, g) immunoblotting for AR-V7 or AR-FL (full-length) and by (H) qRT-PCR analysis of indicated genes.
