Figure 6
Blocking the lipid synthesis pathways prevented cell cycle progression in C4-2 CRPC cells. (a) qRT-PCR analysis for indicated genes in C4-2 cells treated with DHT for 24 h. (b–d) C4-2 cells were treated with DHT alone and/or simvastatin (pretreat 4h) for 24 h, followed by (b) immunoblotting for p-S6, (c) qRT-PCR for PSA mRNA expression, and (d) BrdU incorporation assay. (e) C4-2 cells grown in medium with 8%CSS plus 2%FBS or 10%CSS were treated with simvastatin for 24 h and followed by flow cytometry cells cycle analysis.
