Figure 1 | Oncogene

Figure 1

From: SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/β-catenin signaling through stabilization of the WNT–receptor complex

Figure 1

SPARCL1 is downregulated in OS owing to epigenetic silencing by promoter DNA methylation. (a) SPARCL1 expression patterns in OS cell lines (Saos-2, U-2OS and MNNG-HOS) and normal osteoblast cell line (hFOB1.19) by western blotting. (b) The IHC staining of SPARCL1 in OS human osteosarcoma TMA (n=40, duplicate cores per case). (c) Statistical analysis of IHC based on the protein expression level in 40 OS cases. (d) The mRNA expression of SPARCL1 was evaluated by real-time qPCR in OS cell lines (Saos2, U-2OS and MNNG-HOS) and osteoblast cell line (hFOB1.19) treated with vehicle, DAC, TSA or DAC plus TSA (n=3). Values are means±s.d., *P<0.05; ns indicates no significance. (e) Bisulfite-sequencing results of human osteoblast cell line hFOB1.19 and three OS cell lines (Saos2, U-2OS and MNNG-HOS). Five CpG sites were sequenced. Open circles indicate unmethylated and solid circles represent methylated CpG dinucleotides. (f) Total methylation rates of SPARCL1 promoter in the human osteoblast cell line hFOB1.19 and three OS cell lines (Saos2, U-2OS and MNNG-HOS) (n=1) are shown.

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