Figure 5 | Oncogene

Figure 5

From: SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/β-catenin signaling through stabilization of the WNT–receptor complex

Figure 5

Correlation between SPARCL1 expression and M1 macrophages infiltration in OS. (a) Pearson correlation analysis between SPARCL1 and TAMs by analyzing OS gene expression database (http://hgserver1.amc.nl). Ibal1, CD11b and CD68 are total TAM-associated makers; Fizz1, CD163 and iNOS are M2-associated makers; M1-associated makers are HLA-DR and CD11c. (b) Representative images of IHC staining of SPARCL1 and HLA-DR in 40 OS cases. Two cases of negative expression of SPARCL1 and HLA-DR were shown in upper panel and lower panel revealed two cases of positive expression of SPARCL1 and HLA-DR. Scale bars, 100 μm. (c) Spearman correlation analysis between SPARCL1 score and number of M1 HLA-DR+ macrophages based on the IHC staining of 40 osteosarcoma cases (r=0.4781, P=0.0018) (HPF, high power field). (d) The more infiltration of macrophages in SPARCL1-overexpressing metastatic lung nodules compared with vector group. Representative images of IHC staining against HLA-DR in mice lung tissues are shown. Scale bars, 100 μm. (e and f) Kaplan–Meier analysis of overall and metastasis-free survival rate related to the expression of HLA-DR expression in 88 OS cases based on a human osteosarcoma gene expression database (http://hgserver1.amc.nl).

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